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. 2018 Sep 10;8:13545. doi: 10.1038/s41598-018-31787-7

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Misexpression of Lunasin can reduce the mortality rate of elav > Aβ42 flies. (A) A graph comparing the number of flies hatched in Wild-type, elav > Aβ42 and elav > Aβ42 + Lun background validates that Lunasin misexpression along with elav > Aβ42 (elav > Aβ42 + LunGFP) rescues the elav > Aβ42 mortality rate. We counted 200 flies in three independent sets from each background and plotted on a graph. These numbers are significant based on the calculations of P-values using the two-tailed t- test using Microsoft Excel 2013.