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. 2018 Sep 10;8:13563. doi: 10.1038/s41598-018-31634-9

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Four motifs are conserved in the ES domain across angiosperms. (A,B) Schematic representation of the conserved motifs identified in the ES domain (A) and their sequence and possible function (B). (C,D) Alignment of ES domain from different angiosperm species (C) and their phylogenetic tree (D). Motif colour code: red, NLS; yellow, Motif 1A and 1B; blue, Ser patch; purple, Motif 2. (E) Alignment between ETT/ARF3 and ARF4 highlighting conservation of NLS, Motif 1A and 1B and Motif 2 between the two proteins.