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. 2018 Sep 10;8:13563. doi: 10.1038/s41598-018-31634-9

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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ETT 388–594 is the auxin-responsive domain. (A) Schematic representation of ETT variants and their behaviour in Y2H assay in combination with IND and in the absence and presence of auxin at different concentrations. Clones EP1, 2 and 3 were isolated from a random mutagenesis analysis of the ES domain; clones called ES_SP, referring to the Ser patch, are serine to threonine mutations introduced by site-specific mutagenesis. (B–E) Mapping and Scanning electron microscopy images of gynoecium of annotated A. thaliana wild-type (B) and ett mutant: arf3-1 (D), arf3-2 (E) and ett-3 (F). (G–J) Mapping and optical microscopy images of gynoecium of Brassica rapa wild type (G) and ett mutant: BraA.ett.a Stop220 (I) and BraA.ett.a Gln480Stop (J). Abbreviations: rp, replum; sg, stigma; st, style, va, valves. Scale bars: 100 μm (B,D,E), 1 mm (G,I,J).