Fig. 2.

HRD1 inhibition protects mice from HFD-induced obesity and fatty liver disease. a Body weights of WT and HRD1^Alb mice fed a HFD were measured every 2 weeks. Liver (b) and fat (c) weights from WT and HRD1^Alb mice fed a HFD for 14 weeks (n = 6 for each group). H&E (d) and Oil Red O (e) staining of the liver from b (n = 6 for each group). Glucose tolerance assay (f) and insulin tolerance assay (g) were performed on mice after 14 weeks of a HFD (n = 6 for each group). h Serum TG and cholesterol levels from WT and HRD1^Alb mice after 14 weeks of a HFD under the refed condition (n = 6 for each group). i Hepatic gene expression of Srebp1 and Scd1 in WT and HRD1^Alb mice after 14 weeks of a HFD under the refed condition (n = 6 for each group). j Food intake, body weight ratios, and activities of WT and HRD1^Alb mice after 14 weeks of a HFD (n = 4 for each group). k Energy expenditure of WT and HRD1^Alb mice after 14 weeks of a HFD (n = 4 for each group). The data are representative of three independent experiments (mean ± s.d.). *P < 0.05. **P < 0.01 by unpaired Student’s t test