Fig. 3.

Identification of potential E3 ligase HRD1-interacting protein substrates by AP-MS. a Flow chart for the proteomic identification of HRD1-binding proteins. b Venn diagram of high-confidence proteins derived from the COMPASS and SAINT software. c A correlation matrix based on spectral counts of each protein was constructed for the three repeats of HRD1 purification and two repeats of control samples using normal IgG. Pairwise Pearson correlation scores were measured. d Biological process analysis of the functions of HRD1-binding proteins. e GO localization analysis of the HRD1-binding proteins. f Validation of the interactions between HRD1 and potential HRD1-binding proteins from proteomic screening. g Endogenous interaction between HRD1 and screened proteins from f. h C-terminus of HRD1 mediates the interaction with CPT2, RMND1, and ENTPD5. Data are representative of three independent experiments