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. 2018 Sep 10;9:3678. doi: 10.1038/s41467-018-06139-8

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — Characterization of ZmGa1P biochemical properties. a Immuno-staining the pollen tubes of SDGa25 (Ga1-S) and J66 (ga1) using the anti-ZmGa1P antibody. Scale bar = 10 μm. b Kinetic analysis of PME activity of secretory proteins in the fraction 8 of SDGa25. Error bars represent the mean ± SD of three replicates. c Split-luciferase complementation assay showing the interaction between ZmGa1P and the PME domain (CD) of ZmPME10-1 in N. benthamiana leaves infiltrated with the construct combinations shown above. Scale bar = 1 cm. d Immuno-detecting highly methylesterified pectins in the longitudinally sectioned pollen tubes of near isogenic lines with LM20 antibody. Scale bars = 10 μm. FITC, Fluorescein isothiocyanate. e Quantification of the fluorescent signal intensity of immune-examinations, to show the varied pectin DM on ga1 pollen tube walls growing in Ga1-S silks. Error bars represent the mean ± SE (n > 15 pollen tubes). a and b indicate that the means differ according to analysis of variance and Tukey’s test (P < 0.01)