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. 2018 Sep 11;6:26. doi: 10.1038/s41413-018-0027-6

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — Subchondral bone loss in the temporomandibular joint condyle. a Representative µCT images and quantitative analysis showed significant bone loss at 8 wks after surgery in the temporomandibular joint condyle of rats. b Representative µCT images and quantitative analysis showed increased bone loss in the temporomandibular joint condyle of ageing mice. Scale bar = 300 μm. BV/TV (%), bone volume fraction; Tb.Sp (mm), trabecular separation. c, d TRAP staining and immunohistochemical (Osterix and p-Smad2/3) staining of the temporomandibular joint subchondral bone from control and TMD rats (c) and ageing mice (d). Quantitative analysis of TRAP and IHC staining is shown on the right. Scale bar = 20 μm. n = 6 per group. *P < 0.05, one-way ANOVA followed by Tukey’s test. All data are expressed as the mean ± s.d