Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 Sep 4;9:2008. doi: 10.3389/fimmu.2018.02008

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2018 Avendaño-Ortiz, Maroun-Eid, Martín-Quirós, Lozano-Rodríguez, Llanos-González, Toledano, Gómez-Campelo, Montalbán-Hernández, Carballo-Cardona, Aguirre and López-Collazo.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

PMC Copyright notice

The groups of patients with sepsis exhibit different states of activation after ex vivo challenge. (A) Blood samples from patients with sepsis (n = 85) and healthy volunteers (HV, n = 15) were stimulated or not with LPS (5 ng/mL, 3 h) ex vivo. Then, mean intensity of fluorescence (MIF) of HLA-DR on the gate of CD14⁺ cells was analyzed by FACS. Folds after LPS challenge are shown in patients classified according to their oxygen saturation and HV. (B) PBMCs were isolated from patients with sepsis (n = 85) and HV (n = 15), labeled with CFSE and stimulated or not with PWD (2.5 μg/mL) for 5 days. Then, proliferation of CD3⁺ cells was analyzed by FACS. Percentages of proliferation are shown in patients classified according to their oxygen saturation and HV ^*p < 0.05 using a Student's t-test.