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. 2018 Sep 4;9:2041. doi: 10.3389/fmicb.2018.02041

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Copyright © 2018 Llamas-Arriba, Pérez-Ramos, Puertas, López, Dueñas and Prieto.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Detection of plasmids of P. ethanolidurans CUPV141 and CUPV141NR strains and of P. parvulus 2.6. (A) Detection of the gtf gene by Southern blot hybridization. Left, analysis in a 0.7% agarose gel of plasmids preparations of LAB strains and of E. coli V517. Right, hybridized membrane of samples transferred from the agarose gel. (B) Depicts the calibration curve for plasmid size determination. Symbols: plasmids from E. coli V517 (◊), P. ethanolidurans (♦) and P. parvulus (♦) strains. (C) Analysis in 0.7% agarose gel of gtf PCR amplicons obtained with genomic DNA from CUPV141 and CUPV141NR strains. Smart Ladder, molecular weight standard.