Figure 5. Linc00511 was associated with miR-15a-3p.

(A) Prediction for miR-15a-3p-binding elements on linc00511. The red nucleotides (target sites) were deleted in the mutant constructs. (B) The luciferase activity in BIU87 cells were detected by co-transfected with miR-15a-3p and luciferase reporters containing si-NC, si-linc00511 and si-linc00511-mut. Data are presented as the relative ratio of luciferase activity to Renilla luciferase activity. (C) RT-PCR detection of miR-15a-3p expression, showing the negative correlation between linc00511 and miR-15a-3p expression levels in bladder cancer tissues (Spearman’s correlation, r² = 0.59). **P<0.01 compared with NC.