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. 2018 Aug 29;38(4):BSR20180547. doi: 10.1042/BSR20180547

Figure 4. PTEN is the functional downstream effector of miR-216a.

Figure 4

(A) The mRNA expression of PTEN in SKOV3 cells transfected with PTEN overexpression plasmid was determined by qPCR. (B) Cell viability of SKOV3 cells transfected with miR-216a or miR-216a and PTEN was determined by MTT assay. (C) Cell viability of SKOV3 cells transfected with miR-216a or miR-216a and PTEN was determined by soft agar assay. (D) The mRNA levels of PTEN in SKOV3 and SKOV3 CR cells were determined by qPCR. (E) The mRNA levels of PTEN in SKOV3 cells treated with cisplatin (4 μg/ml) for different time were determined by qPCR. (F) Cell viability of SKOV3 cells transfected with or without PTEN and treated with different concentration of cisplatin was determined by MTT assay. (G) Cell viability of SKOV3 cells transfected with miR-216a and/or PTEN and treated with different concentration of cisplatin was determined by MTT assay. **P<0.01; ***P<0.001.