Figure 4.

PDGF-BB promotes osteogenic relative protein expression via the Src/JAK2 signaling pathway. (A) MC3T3-E1 cells were treated with BSA (25 ng/ml), PDGF-BB (25 ng/ml), PDGF-BB (25 ng/ml)+AG1295 (20 µmol/l) and PDGF-BB (25 ng/ml)+SU6656 (2 µmol/l), in osteogenic media for 3 days or (C) 7 days. (B and D) Densitometric analysis of the associated bands were expressed as the relative optical density of the bands, corrected using β-actin as the control (normalization bands). Data are presented as the mean ± standard deviation (n=3). ***P<0.001, as indicated. PDGF-BB, platelet-derived growth factor-BB; JAK2, Janus kinase 2; NS, not significant; BSA, bovine serum albumin; Runx2, Runt-related transcription factor 2; Col1α1, collagen type 1α1.