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. 2018 Aug 3;18(4):3719–3726. doi: 10.3892/mmr.2018.9351

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Copyright: © Liu et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 5. — PDGF-BB promotes MC3T3-E1 cell differentiation and proliferation via the Src/JAK2 signaling pathway. (A) Images of M3T3-E1 cells treated with BSA (25 ng/ml), PDGF-BB (25 ng/ml), PDGF-BB (25 ng/ml)+AG1295 (20 µmol/l), PDGF-BB (25 ng/ml)+SU6656 (2 µmol/l) and PDGF-BB (25 ng/ml)+AG490 (10 µmol/l) in osteogenic media stained by alkaline phosphatase at 3 and 7 days. (B) The OD values at 450 nm were then measured for 72 h, and the data were expressed as the mean ± standard deviation (n=3) with six replicates. ***P<0.001 vs. PDGF-BB+AG1295; ^###P<0.001 vs. PDGF-BB+SU6656; ^$$$P<0.001 vs. PDGF-BB+AG490. PDGF-BB, platelet-derived growth factor-BB; BSA, bovine serum albumin; JAK2, Janus kinase 2; OD, optical density; CCK, Cell Counting kit; ALP, alkaline phosphatase.