Figure 6.

PDGF-BB promotes MC3T3-E1 cell differentiation and mineralization via the Src/JAK2 signaling pathway. (A) M3T3-E1 cells were treated with BSA (25 ng/ml), PDGF-BB (25 ng/ml), PDGF-BB (25 ng/ml)+AG1295 (20 µmol/l), PDGF-BB (25 ng/ml)+SU6656 (2 µmol/l) and PDGF-BB (25 ng/ml)+AG490 (10 µmol/l) in osteogenic media and stained with Alizarin Red at (B) 14 and (D) 21 days. (B and C) Quantification of Alizarin Red S staining with Hexadecylpyridinium Chloride Monohydrate; the amount of released dye was quantified by thermoMUltiskan EX plate reader at 562 nm. *P<0.05, **P<0.01 and ***P<0.001, as indicated. PDGF-BB, platelet-derived growth factor-BB; JAK2, Janus kinase 2; OD, optical density; BSA, bovine serum albumin.