Fig. 1.

VWF A3 and DDR2 binding to crosslinked collagen films. Wells were coated with collagen films and subsequently treated with various concentrations of EDC and NHS in 70% EtOH, ranging from 1% to 100% of the standard concentration (100%) for 2 h at room temperature. VWF-A3-GST (A) at 5 μg/ml for 1 h or DDR2-Fc (B) at 1 μg/ml for 2 h were incubated on crosslinked collagen films at room temperature, and detected with anti-GST and anti-Fc antibodies respectively. Additional wells without collagen were coated with III-23 or GPP10 for positive and negative control respectively at 10 μg/ml in AcOH 0.01 M overnight. These graphs show mean values with standard error of pooled results of three independent data sets (n = 3 for each condition within each experiment; 1-way ANOVA, p < 0.01). Overall, VWF and DDR2 binding decreased as the degree of film crosslinking increased. Statistical significance is shown compared to 0% EDC/NHS for all other EDC/NHS concentrations.