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. 2018 Nov;182:21–34. doi: 10.1016/j.biomaterials.2018.07.050

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© 2018 The Authors

This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).

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Fig. 6 — Western blot of HEK-293 cell lysates transfected with DDR2-Flag and incubated for 90 min on 100% EDC/NHS crosslinked films derivatised with peptides at 5 μg/ml and left in the dark or exposed to UV light for 5 min. Collagen films were non-crosslinked (Lane 6); or 100% crosslinked with no peptides (Lane 5); with VWFIII_Nle no UV (Lane 4); with VWFIII_Nle after UV (Lane 3); with Diaz-ES-VWFIII_Nle no UV (Lane 2); or with Diaz-ES-VWFIII_Nle after UV (Lane 1). DDR2-Flag and DDR2 phosphorylation were detected on a single membrane. (A) DDR2-Flag staining with anti-Flag and anti-mouse 800CW detected at 800 nm; (B) DDR2 phosphorylation staining with pY740 and anti-rabbit 680 RD detected at 700 nm; (C) Merge. Western blots show co-localization of 700 nm and 800 nm fluorescence on a single membrane between 125 and 130 kDa, confirming that antibodies used in our experiments target specifically DDR2-Flag and DDR2 phosphorylation as expected.