Table 3.
Metal content determination by ICP-MS after exposure of A2780 and A2780cisR cells with compound 1 and cisplatin, incubated in absence and presence of CuCl2 (30 μM) or cimetidine (Cim, 300 μM), recorded after 24 and 72 h incubation.
| Au and Pt content [ng metal/μg protein] | ||||
|---|---|---|---|---|
| 24 h | 72 h | |||
| Treatment | A2780 | A2780cisR | A2780 | A2780cisR |
| COMPOUND 1 | ||||
| [5 μM] | 0.04 ± 0.01 | 0.04 ± 0.01 | nd | nd |
| [10 μM] | 0.12 ± 0.03 | 0.10 ± 0.02 | nd | nd |
| [5 μM] + CuCl2 | 0.08 ± 0.02 | 0.08 ± 0.01 | nd | nd |
| [10 μM] + CuCl2 | 0.48 ± 0.06 | 0.21 ± 0.01 | nd | nd |
| [5 μM] + Cim | 0.04 ± 0.01 | 0.04 ± 0.01 | nd | nd |
| [10 μM] + Cim | 0.26 ± 0.06 | 0.12 ± 0.02 | nd | nd |
| [3 μM] | nd | nd | 0.08 ± 0.01 | 0.05 ± 0.01 |
| [6 μM] | nd | nd | 0.19 ± 0.02 | 0.15 ± 0.01 |
| [3 μM] + CuCl2 | nd | nd | 0.16 ± 0.03 | 0.12 ± 0.01 |
| [6 μM] + CuCl2 | nd | nd | 0.15 ± 0.01 | 0.28 ± 0.03 |
| [3 μM] + Cim | nd | nd | 0.09 ± 0.02 | 0.06 ± 0.02 |
| [6 μM] + Cim | nd | nd | 0.25 ± 0.05 | 0.16 ± 0.01 |
| [15 μM] | 0.07 ± 0.01 | 0.06 ± 0.01 | nd | nd |
| [20 μM] | 0.17 ± 0.04 | 0.08 ± 0.02 | nd | nd |
| [15 μM] + CuCl2 | 0.09 ± 0.01 | 0.07 ± 0.01 | nd | nd |
| [20 μM] + CuCl2 | 0.20 ± 0.03 | 0.13 ± 0.01 | nd | nd |
| [15 μM] + Cim | 0.08 ± 0.02 | 0.06 ± 0.01 | nd | nd |
| [20 μM] + Cim | 0.25 ± 0.02 | 0.10 ± 0.01 | nd | nd |
| [5 μM] | nd | nd | 0.04 ± 0.02 | 0.02 ± 0.01 |
| [10 μM] | nd | nd | 0.07 ± 0.02 | 0.05 ± 0.01 |
| [5 μM] + CuCl2 | nd | nd | 0.05 ± 0.02 | 0.02 ± 0.01 |
| [10 μM] + CuCl2 | nd | nd | 0.09 ± 0.02 | 0.07 ± 0.01 |
| [5 μM] + Cim | nd | nd | 0.02 ± 0.01 | 0.01 ± 0.01 |
| [10 μM] + Cim | nd | nd | 0.05 ± 0.02 | 0.04 ± 0.01 |
The reported values are the mean ± SD of three independent determinations. nd, not determined.