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. 2018 Aug 16;18(4):3611–3618. doi: 10.3892/mmr.2018.9392

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Copyright: © Ye et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 4. — Glt25d1^+/− primary hepatocytes show increased viability loss upon treatment with 1% CCl₄. (A) Morphology of mouse primary hepatocytes following initial plating (magnification, ×100). (B) Morphology of mouse primary hepatocytes following incubation for 4 h (magnification, ×100). (C) Viability of primary hepatocytes evaluated by trypan blue exclusion. The arrow indicates a dead cell (magnification, ×100). (D) Purity of primary hepatocytes identified by PAS staining (magnification, ×400). (E) Effects of 1% CCl₄ treatment for 24 h in primary hepatocytes. *P<0.05; significance determined by Student's t-test. Glt25d1, collagen β (1-O) galactosyltransferase 1; CCl₄, carbon tetrachloride; WT, wild-type.