Figure 3.

PYGB silencing promotes the apoptosis of PC3 cells. PC3 cells were transfected with siRNA negative control (mock group) and PYGB siRNA (si-PYGB group). The cells in control group received no treatment. (A) Flow cytometric analysis was performed to determine the levels of cell apoptosis in PC3 cells. (B) Colorimetry was performed to measure the activity of caspase-3 in PC3 cells. (C) Western blotting was performed to measure the expression levels of PARP, cleaved-PARP and cleaved-caspase-3 in PC3 cells transfected with the siRNA negative control (mock group) and PYGB siRNA (si-PYGB group); the control group was untreated. **P<0.01 vs. mock group. No significant differences were detected between the control and mock groups. PYGB, brain-type glycogen phosphorylase; PARP, poly (adenosine diphosphate-ribose) polymerase; siRNA, small interfering RNA; PI, propidium iodide; FITC, fluorescein isothiocyanate.