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. 2018 Sep 10;8:13541. doi: 10.1038/s41598-018-31994-2

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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(a) Investigation of different antigen incubation time on GPC-1 biosensor. (b) Modified self-assembled monolayer system (3-MPA & DTT) based GPC-1 biosensor (Biosensor 1) on DPV measurements of multiple concentrations of GPC-1 antigen in undiluted human serum. (c) SAMSA modified antibody based GPC-1 biosensor (Biosensor 2) on DPV measurements of multiple concentrations of GPC-1 antigen in undiluted human serum. (d) The calibration curve based on Biosensor 1 and Biosensor 2. (e) Exhibition of change of current response at different concentrations of GPC-1 antigens based on two different biosensors. (f) Interference study based on HE4 antigen.