Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 Sep 10;8:13489. doi: 10.1038/s41598-018-31657-2

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

p300G422S-KI increases adult neurogenesis at the age of 3 months by reducing cell death of newborn neurons. (a) Representative images of hippocampal sections from 3-month p300G422S-KI (homologous p300G422S-KI) and their WT, sacrificed 30 days after BrdU injections, and stained for BrdU (green) and NeuN (red). Arrows represent BrdU+/NeuN+ neurons. Scale bar = 20 µm. (b) Quantitative analysis of the total number of BrdU+/NeuN+ newborn neurons in the hippocampi from 3 months WT and p300G422S-KI, as shown in (a). (c) Representative fluorescence images of hippocampal sections from 3 months WT and p300G422S-KI mice, sacrificed one day after BrdU injections, stained for BrdU (green) and Ki67 (red). Arrows denote BrdU+/Ki67+ proliferating cells. Scale bar = 20 μm. (d) Quantitative analysis of the total number of BrdU+/Ki67+ proliferating cells in the hippocampi from 3 months p300G422S-KI and their WT littermates. (e) Representative images of hippocampal sections from 3 months WT and p300G422S-KI mice, sacrificed 12 days after BrdU injections, stained for BrdU (green) and NeuN (red). Arrows represent BrdU+/NeuN+ neurons. Scale bar = 20 µm. (f) Quantitative analysis of the total number of BrdU+/NeuN+ neurons in the hippocampi from WT and p300G422S-KI mice (3-month) as shown in (e). (g) Representative images of hippocampal sections from 3-month WT mice, stained for cleaved caspase 3 (CC3) (green) and DCX (red). Arrows denote CC3+/DCX+ cells. Scale bar = 10 μm. (h) Quantitative analysis of the total number of CC3+/DCX+ cells in the hippocampi from 3 months p300G422S-KI and their WT littermates. (i) Representative images of hippocampal sections from 6 months WT and p300G422S-KI mice, sacrificed 12 days after BrdU injections, stained for BrdU (green) and NeuN (red). Arrows represent BrdU+/NeuN+ neurons. Scale bar = 20 µm. (j,k) Quantitative analysis of total number (j) and the proportion (k) of BrdU+/NeuN+ cells in the hippocampi from WT and p300G422S-KI mice (6 months) as shown in (i). The boxed areas were shown at higher magnification on the right panels in (a,e,i). *p < 0.05; ***p < 0.001, n = 4 animals for each group.