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. 2018 Sep 4;9:2019. doi: 10.3389/fimmu.2018.02019

Figure 6.

Figure 6

Internalizable IgG-mediated production of IL-8 and TNF-α occurs via NF-κB activation through the MAPK signaling pathway. (A) Pharmacological inhibition of cytokine production by THP-1 cells exposed to 3D8 IgG. Cells were pre-treated for 1 h with 0.5 μM 5z-7-oxozeaenol, 200 nM IKK inhibitor VII, 10 μM SB202190, 50 μM PD98059, or 20 μM SP600125 prior to exposure to 5 μM 3D8 IgG. The amount of IL-8 and TNF-α in the culture supernatant was measured using ELISA kits. (B) Luciferase assay to detect activation of the NF-κB promoter. THP-1 NF-κB reporter cells, which were established using a lentiviral system, were pre-treated for 1 h with 0.5 μM 5z-7-oxozeaenol and then incubated with 5 μM 3D8 IgG for 24 h. Cells were harvested, and luciferase assays were performed. All p values were calculated using a two-tailed Student's t-test (***p < 0.001, vs. treatment with 3D8 IgG alone).