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. 2018 Sep 4;9:2022. doi: 10.3389/fimmu.2018.02022

Figure 2.

Figure 2

Induction of type I IFN by O. tsutsugamushi infection through RIG-I/MAVS and STING. (A) MEFs derived from wild type (WT), MAVS-deficient (MAVS KO), RIG-I-deficient (RIG-I KO), or STING-deficient (STING KO) mice were infected with O. tsutsugamushi and the relative expression of IFN-β and TNF-α mRNA, as well as type I IFN bioactivity, were analyzed at 4 h after infection as describe in Figure 1. (B) BMDMs derived from wild type (WT), MyD88-deficient (MyD88 KO), or TRIF-deficient (TRIF KO) mice were infected with O. tsutsugamushi and the relative expression of IFN-β and TNF-α mRNA, as well as type I IFN bioactivity, were determined at 4 h after infection as described in Figure 1. Data represent mean ± SD of three independent experiments. Statistical significance was determined by two-tailed Student's t-test with 95% confidence interval for comparisons of values between wild type and KO cells after infection with O. tsutsugamushi. ***p < 0.001. RLU, relative luciferase unit. White box, uninfected (U.I.); black box, infected (Inf.).