Table 2.

The purification and characteristics of the protein samples that are used in this study.

	Sub domain of	Strain	Tag	Purification 1	Purification 2	Purification 3	Final buffer	Quantification method	Concentration (mg/mL)	% disorder	ε280 nm (M−1 cm−1)	Molecular weight	References
AF1	AR	BL21*	His6 and smt3	Histrap	Histrap	GF S200 16/100	TBS + 0.5 mM TCEP	UV	10.0	51	30,370	33,887	Unpublished
EM	–	BL21	His6	Histrap	GF S7516/60	–	PBS	Weight	7.8	100	1,490	12,150	Unpublished
ERD10	–	BL21*	NA	Q FF HiTrap	GF s200 16/60	–	PBS	Weight	7.8	100	2,980	29,547	Kovacs et al., 2008
ERD14	–	BL21*	NA	DEAE FF HiTrap	GF s200 16/60	–	PBS	Weight	7.3	100	1,490	20,786	Kovacs et al., 2008
hCSD1	calpastatin	BL21-AI	–	DEAE Hitrap	GF S7516/60	–	30 mM MOPS +1 mM TCEP	UV	7.0	100	4,470	14,764	Nguyen et al., 2017
ID1	CBP	BL21*	His6, N'-terminal	Ni2+-affinity	ionexchange	GF S200 increase	TBS + 0.05 mM TCEP, 5% glycerol	UV	12	99.7	2,980	38,329	Unpublished
ID5	CBP	BL21*	His6	Nickel	GF S200 26/60	–	PBS	UV	9.0	100	4,470	38,291	Contreras-Martos et al., 2017
α-synuclein	–	BL21	N/A	DEAE Hitrap	GF S75 26/60	–	TBS	UV	5.3	40	5,960	14,460	Huang et al., 2005
β-casein$	–	–	–	–	–	–	–	Weight	10.6	38.5	11,460	25,107	-
DBD	AR	BL21*	His6 smt3	Histrap	Heparin	GF S75 16/60	TBS + 0.5 mM TCEP	UV	10.0	0	7,450	10,905	Unpublished
BSA$	–	–	–	–	–	–	–	Weight	10.0	3.9	42,925	69,293	–
Hemoglobin$	–	–	–	–	–	–	–	Weight	10.2	0	47,900	62,275	–
Hint1	–	BL21-AI	His6	Nickel	GF S200 16/60	–	PBS	UV	9.5	1.6	8,480	15,369	Unpublished

Protein were obtained from either commercial sources (

^$) or prepared as recombinant proteins in house. Globular proteins are underlined. For in house purified proteins, the table details major steps of the purification pipeline and references to more detailed protocols when appropriate. The protein characteristics like percentage of intrinsic disorder, the molecular extinction coefficient at 280 nm and the molecular weight are also included.

^*refers to E. coli BL21 Star strain.