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. 2018 Sep 4;9:354. doi: 10.3389/fgene.2018.00354

FIGURE 3.

FIGURE 3

Effects of dKDM2 over-expression on methylation levels of H3K4 and H3K36. (A) The levels of H3K36me1/2/3 and H3K4me1/2/3 in dKdm2-overexpressed larvae at the L3 wandering stage. The detailed genotype for “Ub > dKdm2+” line is “w1118; Ubi-Gal4/UAS-dKdm2-eGFP; +.” (B) Quantification of the results of Western blots from three independent biological repeats using a Li-Cor Odyssey Infrared Imaging system. ∗P < 0.05 based on Student’s t-test. (C–E) Immunostaining of wing discs with wild-type dKdm2 overexpressed in the posterior half of the discs (genotype∖n-Gal4/UAS-dKdm2-eGFP) with antibodies against H3K36me1 (C), H3K36me2 (D), and H3K36me3 (E). Images in (C′–E′) show the GFP signals, and (C″–E″) are merged images.