FIGURE 4.

dKDM2 is required for circadian behavior rhythms. (A) Period of circadian locomotor rhythms of the dKdm2 mutants. (B) Effects of dKdm2 overexpression on the period of circadian locomotor rhythms. Error bars indicate SD; the detailed genotype of “TG4/+” is “tim-GAL4, UAS-dicer2/+”; “PG4/+” is “Pdf-GAL4, UAS-dicer2/+”; and “PG80” is “Pdf-GAL80.” Each genotype is compared to its driver or UAS line as the controls. For the data shown in (A) and first three columns in (B), ^∗∗∗∗p < 0.0001, as determined by using Tukey’s multiple comparison test after one-way analysis of variance. For the rest of the data shown in (B), ^∗∗∗∗p < 0.0001, based on t-test. Number of animals analyzed is included in Supplementary Table S1. (C,D) Locomotor behavior under light dark (LD) cycle and constant darkness (DD). Representative double plotted actograms showing average activity of flies during 3 days of LD and 5 days of DD for controls, dKdm2 mutants (C) and dKdm2 overexpression (D) in PDF positive sLNvs using the Pdf-GAL4 driver. The light phase is shown in white color, and the dark phase is shown in gray. Representative confocal images showing the effects of dKdm2 mutation on the levels of PER (E) and CLK (F) in sLNvs. Flies were entrained under 3 days of LD, and brains were dissected at CT0 (CT = circadian time) in the first day of DD. Fly brains were dissected at CT4. Scale bars in (E,F): 10 μm.