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. 2018 Jul 18;4:79. doi: 10.1038/s41420-018-0081-2

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — a There is absence of p21 immunoreactivity in nonirradiated dentate gyrus whereas p21 nuclear immunostaining is apparent in both granule and subgranular cells at 9 weeks after 5 Gy (p21, red; DAPI, blue). b A type-1 cell (arrow) demonstrates p21 nuclear immunoreactivity after irradiation (nestin, green; GFAP, white; p21, red; DAPI, blue). c Some type-2a (Tbr2+/DCX−, arrowhead), type-2b (arrows, Tbr2+/DCX+), and type-3 neural progenitors (open arrow, Tbr2−/DCX+) demonstrate p21 nuclear immunoreactivity (Tbr2, green; DCX, white; p21, red; DAPI, blue) after irradiation