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. 2018 Sep 4;9:1994. doi: 10.3389/fimmu.2018.01994

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Copyright © 2018 Li, Huang, Jiang, Chen, Zhang, Wei, Chen, Lian, Bian, Miao, Peng, Fang, Wang, Tang, Gershwin and Ma.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Macrophage polarization induced by activated MAIT cells in vitro. (A) Representative flow cytometry scatter plots of M1 (HLADR⁺CD80⁺CD86⁺) and M2 (CD163⁺CD206⁺) phenotype in CD14⁺ monocytes treated with unactivated MAIT cells or activated MAIT cells. (B) A higher M2/M1 ratio in macrophages co-cultured with activated MAIT cells (n = 5). (C) No significant difference in M2/M1 cells ratio among macrophages treated with activated MAIT cells between co-culture system and transwell experiments (n = 4). (D) Comparison of IL-4 production in unactivated and activated MAIT cells (n = 4). Data were analyzed with paired t-test and Mann–Whitney U-test. *P < 0.05, **P < 0.01, ns: no statistical significance.