Figure 2.

Effects of Sirt3 on cisplatin-induced macrophage infiltration. (A) Representative immunofluorescence staining for F4/80 in kidneys from WT and Sirt3KO mice treated with vehicle or cisplatin. Nuclear staining was performed using DAPI. Scale bar, 25 µm. (B) Bar graph of the number of F4/80-positive cells in the kidneys of WT and Sirt3KO mice. A total of 10 randomly selected, nonoverlapping fields were quantified (n=10 in each group; magnification, ×400). *P<0.05 vs. Veh; ^#P<0.05 vs. WT Cisplatin. Data are expressed as the mean ± standard deviation. Sirt3KO, NAD⁺-dependent protein deacetylase 3 knockout mice. Veh, vehicle; WT, wild type; Sirt3, Sirtuin 3; KO, knockout; F4/80, adhesion G protein-coupled receptor E4.