Figure 4.

Effects of Sirt3 on cisplatin-induced tubular MCP-1 expression. (A) Representative immunohistochemistry staining for MCP-1 in the kidneys of WT and Sirt3KO mice treated with vehicle or cisplatin. Scale bar, 100 µm. (B) Bar graph demonstrated area fraction of MCP-1 in WT and Sirt3KO mice treated with vehicle or cisplatin. A total of 10 randomly selected, nonoverlapping fields at a magnification of ×200 were quantified (n=10 in each group). IgG control antibody was used for negative control for staining. **P<0.01 vs. Veh; ^##P<0.01 vs. WT cisplatin. Data are expressed as the mean ± standard deviation. MCP-1, monocyte chemoattractant protein-1; Veh, vehicle; WT, wild type; Sirt3, Sirtuin 3; KO, knockout.