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. 2018 Sep 4;12:295. doi: 10.3389/fncel.2018.00295

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Copyright © 2018 Pyrski, Tusty, Eckstein, Oboti, Rodriguez-Gil, Greer and Zufall.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Cav2.1 staining reveals a unique subset of glomeruli in the MOB. (A) Cav2.1 immunoreactivity (red) exemplified in a whole-mount preparation of the left and right MOB (dorso-caudal view) of an adult B6 mouse. Cav2.1+ axon bundles (arrows) terminate into several individual glomeruli (arrowheads). Large Cav2.1+ glomeruli reside in the caudal aspect of the MOB, close to the accessory olfactory bulb (AOB). Frequently, two glomeruli reside next to each other (brackets). Staining is absent in the AOB. (B) Negative control reaction using peptide blocking is devoid of Cav2.1 staining (dorso-caudal view and right bulb). (C) Sagittal section (14 μm) at about the MOB midline depicting four individual Cav2.1+ glomeruli (arrows 1–4) and their afferent axonal fibers (arrowheads). The inset (bottom) shows a higher magnification of two glomeruli (2, 3) and axonal projections from the nerve layer of the MOB. Nuclei staining with Hoechst dye (blue) verifies glomerular boundaries. (D) Coronal section (14 μm) of the posterior MOB showing two large Cav2.1+ glomeruli medial and lateral to the AOB. The inset at the bottom depicts a higher magnification of the medial glomerulus. (E) Cav2.1+ glomeruli (arrows) are already evident at postnatal day 7 (P7) including afferent axons (arrowheads, inset, and bottom). (A–E) Orientations are as depicted by arrows indicating a, anterior; p, posterior; d, dorsal; v, ventral; l, lateral; m, medial; AON, anterior olfactory nucleus. Scale bars (A–E) overviews, 500 μm; (C,D) insets, 100 μm; (E) inset, 50 μm.