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. 2018 Sep 4;12:295. doi: 10.3389/fncel.2018.00295

FIGURE 3.

FIGURE 3

Cav2.1 is localized to presynaptic OSN axon terminals. Confocal images showing immunoreactivity (red) for (A) OMP, (B) vGlut2, (C) Map2, and (D) vGlut1 in combination with Cav2.1 (green) in coronal MOB sections of adult B6 mice. (A,B) The merged images illustrate that the presynaptic markers OMP and vGlut2 colocalize with Cav2.1 yielding the yellow fluorescence signal. (C,D) In contrast, the post-synaptic dendritic markers Map2 and vGlut1 do not colocalize with Cav2.1 in the glomerular neuropil. This is illustrated in the magnified merge at the bottom (boxed area) showing separate red and green fluorescence signals. Scale bars (A–D) 40 μm, inset magnifications, 20 μm.