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. 2018 Sep 4;12:295. doi: 10.3389/fncel.2018.00295

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Copyright © 2018 Pyrski, Tusty, Eckstein, Oboti, Rodriguez-Gil, Greer and Zufall.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Cav2.1+ glomeruli receive afferent input from a specific subset of OSNs. (A) Cav2.1 immunoreactivity (red) and endogenous GFP fluorescence in a whole-mount MOB preparation (dorsal-caudal view) of an adult OMP-GFP mouse. Cav2.1 and GFP colocalize in individual glomeruli (arrows). Coronal MOB section (14 μm) showing that Cav2.1+ glomeruli (red) colocalize with Pde4a (green) (B) and Cnga2 (green) (C). (D,E) Non-canonical markers are absent in Cav2.1+ glomeruli. (D) Whole-mount view (top) showing that Cav2.1+ glomeruli (arrows) reside anterior to the Pde2a+ glomeruli (green) and the AOB. Pde2a and Cav2.1 label separate sets of glomeruli (bottom, 14 μm coronal section). (E) Whole-mount view of the MOB (top) of a GCG-GFP mouse. Cav2.1+ glomeruli (red, arrows) reside anterior to the GFP+ glomeruli (green) that receive axonal input from GGNs. GCG-GFP+ and Cav2.1+ glomeruli are separate sets of glomeruli (bottom, 14 μm coronal section). (F) Coronal MOB section (14 μm) from a Trpc2-IRES-taulacZ mouse stained for Cav2.1 (red) and β-galactosidase (green). Both the AOB and the vomeronasal nerve (VN) but not the Cav2.1+ glomeruli (arrowheads) are positive for β-galactosidase. (G) Sagittal section (14 μm) of the caudal-ventral MOB from a Gucy1b2-IRES-tauGFP mouse. The single GFP+ (green) glomerulus shown is devoid of Cav2.1 staining (red). Hoechst nuclear dye (blue) defines glomerular boundaries in the merged images. Images are representatives of N ≥ 2 mice with N = every second section per mouse. Scale bars (A) 500 μm, (B–E) 100 μm, (F) 200 μm, (G) 50 μm.