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. 2018 Sep 4;12:295. doi: 10.3389/fncel.2018.00295

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Copyright © 2018 Pyrski, Tusty, Eckstein, Oboti, Rodriguez-Gil, Greer and Zufall.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Cav2.1+ OSNs express the canonical markers OMP, Pde4a, and Cnga2. Coronal MOE sections (14 μm) of adult mice labeled for Cav2.1 and different canonical (A) and non-canonical (B) signal transduction molecules. (A) As shown at the left, Cav2.1 (red) and GFP (green) colocalize in mature OSNs (arrows) of OMP-GFP mice. Cav2.1+ OSNs (red) express Pde4a (green, middle, arrows) and Cnga2 (green, right, arrow). (B) Cav2.1 immunoreactivity (arrows) is absent in non-canonical OSNs such as those expressing Trpc2 (red, left, arrowheads), Taar (green, middle, arrowhead) or GC-D (green, right, arrowhead). Images are representatives of (N ≥ 2) mice with N = every 10th section along the anterior-to-posterior extent of the MOE in each mouse. Scale bars: 20 μm.