Figure 3.

Alo induces breast cancer cell apoptosis. (A) Images of apoptotic MCF-7 and MDA-MB-231 cells incubated with vehicle or Alo (0.1, 0.2 and 0.4 mM) for 24 h, as determined by Hoechst 33342 staining (scale bars, 50 µm). (B) MCF-7 and MDA-MB-231 cells exposed to Alo (0.1, 0.2 and 0.4 mM) for 24 h were harvested and analyzed by flow cytometry. The results were expressed as the mean ± standard deviation of three independent experiments and each was performed in triplicate. *P<0.05 and **P<0.01 vs. non-Alo treated group (0 mM Alo). Alo, Aloperine.