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. 2018 Aug 22;18(4):3699–3710. doi: 10.3892/mmr.2018.9419

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Copyright: © Tian et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 8. — Alo inhibits migration and invasion via the Ras signaling pathway in breast cancer cells. (A) MCF-7 and MDA-MB-231 cell non-directional migration was detected by wound healing assay (scale bars, 200 µm; magnification, ×100). (B) MCF-7 and MDA-MB-231 cell directional migration was evaluated by Transwell assay (scale bars, 200 µm; magnification, ×200). (C) MCF-7 and MDA-MB-231 cell invasion was examined by Transwell invasion assay (scale bars, 200 µm; magnification, ×200). (D) Western blotting analyzed the expressions of MMP-2 and MMP-9 following Alo (0.4 mM) or Ras inhibitor (ISIS 2503) treatment for 24 h. Then the band intensity was quantified by ImageJ software. The results were expressed as the mean ± standard deviation of three independent experiments and each was performed in triplicate. *P<0.05, **P<0.01 and ***P<0.001 vs. control group. Alo, Aloperine; MMP, matrix metalloproteinase.