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. 2018 Sep 4;9:2101. doi: 10.3389/fmicb.2018.02101

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Copyright © 2018 Montón Silva, Otten, Biboy, Breukink, VanNieuwenhze, Vollmer and den Blaauwen.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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PBP1b is required for NADA incorporation by LdtD. (A) Phase contrast and corresponding fluorescence images of cells of BW25113, BW25113ΔlpoA, BW25113ΔmrcA (encoding PBP1a), BW25113ΔlpoB, BW25113ΔmrcB (PBP1b), BW25113ΔpbpC (PBP1c), and BW25113ΔcpoB expressing ldtD and labeled with 0.5 mM NADA. Scale bar, 2 μm. The right panel shows the fluorescence profiles per average cell (from 0 to 1000 AU) plotted against normalized cell length (from 0 to 100%). n represents the number of cells analyzed. (B) Concentration of NADA fluorescence (fluorescence signal per μm³ average cell volume). Quantification of the incorporated NADA in the WT strain, BW25113ΔlpoA, BW25113ΔmrcA, BW25113ΔlpoB, BW25113ΔmrcB, BW25113ΔpbpC, and BW25113ΔcpoB strains expressing ldtD. The values are mean ± SD of n number of cells. n is indicated on the fluorescence profiles.