Figure 5.

TSPAN4 is involved in the pathogenesis of gastric cancer. (A) Total RNA was prepared and subjected to reverse transcription-quantitative polymerase chain reaction analysis. GAPDH was used as a control. The results were analyzed using the 2^−Δ∆Cq method. Data are presented as the mean ± standard deviation of three independent replicates, P-values as indicated. (B) Cellular proliferation of control and TSPAN4 shRNA gastric cancer cells was assessed using the BrdU assay. Data are presented as the mean ± standard error of the mean of five experiments. (C) NCI-N87 cells treated with lentivirus-delivered TSPAN4 knockdown were subcutaneously implanted into female athymic nude mice (n=5 per experimental condition). Tumor images were captured on day 20. Scale bar, 1 cm. (D) Tumor growth curves. Data are presented as the mean ± standard error of the mean. *P<0.01 vs. shRNA con. Con, control; TSPAN4, tetraspanin 4; ADCY3, adenylate cyclase 3; LPAR2, lysophosphatidic acid receptor 2; S1PR1, sphingosine-1-phosphate receptor 1; shRNA, short hairpin RNA.