Figure 4.

Effects of SAL on endoplasmic reticulum stress-associated signaling pathways under H/R in H9c2 cardiomyocytes. H9c2 cells were pre-incubated with or without 10 µM SAL for 30 min prior to hypoxia for 4 h and reoxygenation for 12 h. (A) Expression of p-IRE1α and IRE1α and (B) p-PERK and PERK proteins were measured using western blot analysis. β-actin was used as a loading control. Values are expressed as the mean ± standard deviation from three independent experiments. *P<0.05 and **P<0.01 vs. control; ^#P<0.05 and ^##P<0.01 vs. H/R group. SAL, salidroside; H/R, hypoxia/reoxygenation; p-, phosphorylated; IRE1α, inositol-requiring enzyme-1α; PERK, protein kinase RNA-like ER kinase.