Figure 4.

Overexpression of KLF5 inhibited PAN-induced apoptosis of podocytes. Podocytes were treated with PBS (control), PAN (60 µg/ml), NC + PAN or KLF5 + PAN. (A) Cell apoptosis was investigated using flow cytometry in treated podocytes, and the number of apoptotic cells were quantitatively analyzed. (B) mRNA expression levels of Bax, Bcl-2, caspase-3, caspase-8 and caspase-9 were determined via by reverse transcription-quantitative polymerase chain reaction assays. (C) Protein expression levels of Bax, Bcl-2, caspase-3, caspase-8 and caspase-9 were investigated using western blot analyses, and quantitative analysis was performed using base gray values and GAPDH. *P<0.05, **P<0.01 and ***P<0.001. PAN, puromycin aminonucleoside; KLF5, Krüppel-like factor 5; NC, negative control; Bcl-2, B cell lymphoma 2; Bax, Bcl-2 associated X; PI, propidium iodide; FITC, fluorescein isothiocyanate.