Figure 1.

TGF-β effects the expression of DEC1, DEC2, and EMT-related factors. (A) PC-3 cells were untreated, mock-treated (buffer alone), or treated with 2, 5, 10 ng/ml of TGF-β. After 24 h, total RNA was prepared and subjected to RT-PCR analyses of DEC1, DEC2, E-cadherin, N-cadherin, vimentin, and 18S rRNA. PC-3 cells were treated with TGF-β for 24 h. (B) The cells were lysed and the lysates were subjected to western blot analyses of DEC1, DEC2, pSmad2, E-cadherin, N-cadherin, vimentin, and actin.