Figure 2.

(A-H) Opposite roles of DEC1 and DEC2 in regulating EMT induced by TGF-β. PC-3 cells were transfected with the siRNAs (24 h transfection) or the expression plasmids (18 h transfection) of DEC1 and DEC2; subsequently, the cells were incubated with or without TGF-β (10 ng/ml) for an additional 24 h. (A, C, E and G) Total RNA was prepared and subjected to RT-PCR analyses of DEC1 or DEC2, E-cadherin, N-cadherin, vimentin, and 18S rRNA. (B, D, F, and H) Protein was prepared from the cells and subjected to western blot analyses of DEC1 or DEC2, pSmad2, E-cadherin, N-cadherin, vimentin and actin. One representative result of at least three independent experiments with similar results is shown. DEC1, differentiated embryonic chondrocyte gene 1; DEC2, differentiated embryonic chondrocyte gene 2; EMT, epithelial-mesenchymal transition; RT-PCR, reverse transcription-polymerase chain reaction.