Figure 3.

Effects of DEC1/DEC2 on cell morphology and the abundance of cytoplasmic EMT-related factors in PC-3 cells. PC-3 cells were transfected with DEC1 siRNA or DEC2 siRNA, followed by culture with or without TGF-β (10 ng/ml) for 24 h. Cell morphological changes were recorded by bright-phase microscopy (magnification, ×400). The cells were then fixed, incubated with antibodies against E-cadherin, N-cadherin, or vimentin and visualized using an Alexa Fluor 488-conjugated secondary antibody (green). Nuclei were stained with DAPI (blue). One representative image (magnification, ×400) of three independent experiments with similar results is shown. The black arrows indicate fibroblast-like cells. DEC1, differentiated embryonic chondrocyte gene 1; DEC2, differentiated embryonic chondrocyte gene 2; EMT, epithelial-mesenchymal transition.