Table I.
Gene | Product size (bp) | Cycles | Primer sequences |
---|---|---|---|
DEC1 | 534 | 27 | F: 5′-GTCTGTGAGTCACTCTTCAG-3′ |
R: 5′-GAGTCTAGTTCTGTTTGAAGG-3′ | |||
DEC2 | 501 | 27 | F: 5′-CACCTTTGACGTCTTTGGAG-3′ |
R: 5′-GAGAGTGGGAATAGATGCAC-3′ | |||
E-cadherin | 200 | 27 | F: 5′-TGCCCAGAAAATGAAAAAGG-3′ |
R: 5′-GTGTATGTGGCAATGCGTTC-3′ | |||
N-cadherin | 201 | 29 | F: 5′-ACAGTGGCCACCTACAAAGG-3′ |
R: 5′-CCGAGATGGGGTTGATAATG-3′ | |||
Vimentin | 301 | 29 | F: 5′-CTTCGCCAACTACATCGACAA-3′ |
R: 5′-CGCATTGTCAACATCCTGTC-3′ | |||
18s rRNA | 151 | 20 | F: 5′-GTAACCCGTTGAACCCCATT-3′ |
R: 5′-CCATCCAATCGGTAGTAGCG-3′ |
RT-PCR, reverse transcription-polymerase chain reaction; F, forward primer; R, reverse primer.