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. 2018 Sep 4;9:1229. doi: 10.3389/fphys.2018.01229

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Copyright © 2018 Zheng, Zhu, Li, Li, Shen, Chen, Zhong and Deng.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Effect of HEA treatment on fibrosis-related gene and protein expression in ligated kidneys. (A–D) Relative mRNA levels of TGF-β1, α-SMA, collagen I, and Fibronectin expression in kidneys detected by PCR. (E) Representative photographs showing protein expression of TGF-β1, α-SMA, collagen I and Fibronectin in the ligated kidney by western blot analysis. (F–I) Semi-quantitative analyses versus (E). (J,K) The expression of TGF-β1 and α-SMA in ligated kidneys detected by immunohistochemistry. The relative results showed that HEA treatment reduced TGF-β1, α-SMA, collagen I and Fibronectin levels in the left ligated kidney. The data are presented as the means ± SEM. ^∗P < 0.05 and ^∗∗P < 0.01. Scale bar = 100 μM.