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. 2018 Sep 4;9:1229. doi: 10.3389/fphys.2018.01229

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Copyright © 2018 Zheng, Zhu, Li, Li, Shen, Chen, Zhong and Deng.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Effect of HEA on macrophage phenotype in UUO mice. Representative immunofluorescence images of F4/80-labeled macrophages (green) from the sham and UUO groups. M1 macrophages were immunostained with inducible nitric oxide synthase (iNOS) and M2 macrophages were immunostained with IL-10. DAPI labels in the nucleus (blue) (Magnification ×600). (A) HEA blocked the accumulation of M1 macrophages and induced the accumulation of M2 macrophages in UUO kidneys (B) iNOS and IL-10 expression were examined by western blot (C) and semi-quantified by normalization to GAPDH (D,E). The data are presented as the means ± SEM. ^∗P < 0.05 and ^∗∗P < 0.01. Scale bar = 20 μM.