Table 3.
Susceptibility Testing Results for DBOs Alone and in Combination with Cefepime or Meropenem
| strain | 3 | 2 | 1 | avibac-tam | relebac-tam | cefepime | cefepime/3–4a | cefepime/3–8a | cefepime/1–4 | cefepime/1–8 | cefepime/2–4 | cefepime/2–8 | cefepime/avibactam-4 | cefepime/relebactam-4 | mero-penem | mero-penem/3–4 | mero-penem/3–8 | mero-penem/1–4 | mero-penem/1–8 | mero-penem/2–4 | mero-penem/2–8 | mero-penem/avibactam-4 | mero-penem/ relebactam-4 |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Isogenic Strains | |||||||||||||||||||||||
| Escherichia coli DH10B (control) | >16 | <0.12 | <0.12 | 16 | >16 | <0.12 | <0.12 | <0.12 | <0.12 | <0.12 | <0.12 | <0.12 | <0.12 | <0.12 | <0.12 | <0.12 | <0.12 | <0.12 | <0.12 | <0.12 | <0.12 | <0.12 | <0.12 |
| E. coli DH10B pBR322- catI-ICPC-2 | >16 | 0.25 | 0.25 | >16 | >16 | >16 | <0.12 | <0.12 | <0.12 | <0.12 | <0.12 | <0.12 | <0.12 | <0.12 | 8 | <0.12 | <0.12 | <0.12 | <0.12 | <0.12 | <0.12 | <0.12 | <0.12 |
| E. coli DH10B pBC SIC (−) KPC-2 | >16 | <0.12 | <0.12 | >16 | >16 | 0.5 | <0.12 | <0.12 | <0.12 | <0.12 | <0.12 | <0.12 | <0.12 | <0.12 | <0.12 | <0.12 | <0.12 | <0.12 | <0.12 | <0.12 | <0.12 | <0.12 | <0.12 |
| E. coli DHIOB pBC SIC (−) ICPC-3 | >16 | <0.12 | 0.25 | >16 | >16 | 2 | <0.12 | <0.12 | <0.12 | <0.12 | <0.12 | <0.12 | <0.12 | <0.12 | 0.5 | <0.12 | <0.12 | <0.12 | <0.12 | <0.12 | <0.12 | <0.12 | <0.12 |
| E. coli DHIOB pBC SIC (−) PDC-3 | >16 | <0.12 | 0.25 | 16 | >16 | <0.12 | <0.12 | <0.12 | <0.12 | <0.12 | <0.12 | <0.12 | <0.12 | <0.12 | <0.12 | <0.12 | <0.12 | <0.12 | <0.12 | <0.12 | <0.12 | <0.12 | <0.12 |
| E. coli DHIOB pBC SIC (−) OXA-48 | >16 | <0.12 | <0.12 | 16 | >16 | <0.12 | <0.12 | <0.12 | <0.12 | <0.12 | <0.12 | <0.12 | <0.12 | <0.12 | 0.25 | <0.12 | <0.12 | <0.12 | <0.12 | <0.12 | <0.12 | <0.12 | 0.25 |
| A. baumannii AADC | >16 | >16 | >16 | >16 | >16 | 0.25 | 0.5 | 0.5 | 0.5 | 0.5 | 0.25 | 0.5 | 0.5 | 0.25 | <0.12 | <0.12 | <0.12 | <0.12 | <0.12 | <0.12 | <0.12 | <0.12 | <0.12 |
| A. baumannii AADC pWH1266 OXA-23 | >16 | >16 | >16 | >16 | >16 | >16 | 2 | 2 | 16 | 16 | 16 | 16 | >16 | >16 | >16 | 16 | 4 | >16 | >16 | >16 | >16 | >16 | >16 |
| Clinical K. pneumoniae Isolates Producing blaKPC: Colis tin Resistant (ColR) and Colis tin Susceptibile (ColS) | |||||||||||||||||||||||
| K. pneumoniae 660/1568 ColR ICPC-3 | >16 | <0.12 | <0.12 | 4 | >16 | 16 | 0.25 | 0.25 | <0.12 | <0.12 | <0.12 | <0.12 | <0.12 | 0.25 | 2 | <0.12 | <0.12 | <0.12 | <0.12 | <0.12 | <0.12 | <0.12 | <0.12 |
| K. pneumoniae 729/1679 ColR ICPC-2 | >16 | 2 | 0.5 | >16 | >16 | >16 | 0.5 | 0.25 | <0.12 | <0.12 | <0.12 | <0.12 | 0.25 | 0.5 | >16 | <0.12 | <0.12 | <0.12 | <0.12 | <0.12 | <0.12 | <0.12 | <0.12 |
| K. pneumoniae 776/1742 ColR ICPC-2 | >16 | 0.5 | 0.5 | >16 | >16 | >16 | 1 | 1 | <0.12 | <0.12 | <0.12 | <0.12 | 0.5 | 1 | >16 | 0.5 | 0.25 | 0.25 | <0.12 | <0.12 | <0.12 | <0.12 | 0.5 |
| Κ.. pneumoniae 901/1860 ColR ICPC-3 | >16 | >16 | >16 | >16 | >16 | 16 | <0.12 | <0.12 | <0.12 | <0.12 | <0.12 | <0.12 | <0.12 | <0.12 | 16 | <0.12 | <0.12 | <0.12 | <0.12 | <0.12 | <0.12 | <0.12 | <0.12 |
| K. pneumoniae 935/1884 ColR ICPC-3 | >16 | >16 | >16 | >16 | >16 | >16 | 0.25 | <0.12 | <0.12 | <0.12 | <0.12 | <0.12 | <0.12 | <0.12 | >16 | <0.12 | <0.12 | <0.12 | <0.12 | <0.12 | <0.12 | <0.12 | <0.12 |
| K. pneumoniae 977/1916 ColR ICPC-3 | >16 | >16 | 2 | >16 | >16 | 16 | <0.12 | <0.12 | <0.12 | <0.12 | <0.12 | <0.12 | <0.12 | 0.25 | >16 | <0.12 | <0.12 | <0.12 | <0.12 | <0.12 | <0.12 | <0.12 | <0.12 |
| K. pneumoniae 1084/1957 ColR ICPC- 3 | >16 | >16 | >16 | >16 | >16 | >16 | <0.12 | <0.12 | <0.12 | <0.12 | <0.12 | <0.12 | <0.12 | 0.25 | 16 | <0.12 | <0.12 | <0.12 | <0.12 | <0.12 | <0.12 | <0.12 | <0.12 |
| K. pneumoniae 1108/1989 ColR ICPC- 3 | >16 | >16 | >16 | >16 | >16 | >16 | 0.25 | <0.12 | <0.12 | <0.12 | <0.12 | <0.12 | <0.12 | 0.5 | >16 | <0.12 | <0.12 | <0.12 | <0.12 | <0.12 | <0.12 | <0.12 | <0.12 |
| K. pneumoniae 1315/2198 ColR ICPC- 3 | >16 | <0.12 | <0.12 | 4 | >16 | >16 | 0.25 | 0.25 | <0.12 | <0.12 | <0.12 | <0.12 | <0.12 | 0.25 | >16 | <0.12 | <0.12 | <0.12 | <0.12 | <0.12 | <0.12 | <0.12 | <0.12 |
| K. pneumoniae 1398/2291 ColR ICPC- 3 | >16 | 1 | 2 | >16 | >16 | >16 | <0.12 | 0.25 | <0.12 | <0.12 | <0.12 | <0.12 | <0.12 | <0.12 | 16 | <0.12 | <0.12 | <0.12 | <0.12 | <0.12 | <0.12 | <0.12 | <0.12 |
| K. pneumoniae 761/1723 ColS ICPC-3 | >16 | <0.12 | <0.12 | 16 | >16 | 8 | <0.12 | <0.12 | <0.12 | <0.12 | <0.12 | <0.12 | <0.12 | <0.12 | 16 | <0.12 | <0.12 | <0.12 | <0.12 | <0.12 | <0.12 | <0.12 | <0.12 |
| Clinical K. pneumoniae Isolates Producing blaKPC: Colistin Resistant (ColR) and Colistin Susceptibile (ColS) | |||||||||||||||||||||||
| K. pneumoniae 972/1910 ColS KPC-3 | >16 | <0.12 | <0.12 | 4 | >16 | 16 | 0.25 | 0.25 | <0.12 | <0.12 | <0.12 | <0.12 | <0.12 | 0.25 | 8 | <0.12 | <0.12 | <0.12 | <0.12 | <0.12 | <0.12 | <0.12 | <0.12 |
| K. pneumoniae 1354/2241 ColS KPC-3 | >16 | 8 | 8 | >16 | >16 | >16 | <0.12 | <0.12 | <0.12 | <0.12 | <0.12 | <0.12 | <0.12 | <0.12 | 16 | <0.12 | <0.12 | <0.12 | <0.12 | <0.12 | <0.12 | <0.12 | <0.12 |
| K. pneumoniae 440/1360 ColS KPC-2 | >16 | 0.25 | 0.5 | 16 | >16 | >16 | 0.5 | 0.25 | <0.12 | <0.12 | <0.12 | <0.12 | <0.12 | 1 | >16 | <0.12 | <0.12 | <0.12 | <0.12 | <0.12 | <0.12 | <0.12 | <0.12 |
| Clinical Enterobacteriaceae Isolates Producing blaOXA-48 and mutants | |||||||||||||||||||||||
| K. pneumoniae 11978 OXA-48 | >16 | >16 | >16 | >16 | >16 | 16 | 0.5 | 0.5 | <0.12 | <0.12 | <0.12 | <0.12 | 0.25 | 2 | >16 | <0.12 | <0.12 | 1 | <0.12 | <0.12 | <0.12 | 1 | >16 |
| K. pneumoniae CAV 1636 OXA-181 | >16 | >16 | 8 | >16 | >16 | >16 | 0.5 | 0.5 | <0.12 | <0.12 | <0.12 | <0.12 | 0.5 | 4 | >16 | <0.12 | <0.12 | <0.12 | <0.12 | 1 | <0.12 | 1 | 16 |
| K. pneumoniae CAV 1543 OXA-48 | >16 | 1 | 1 | >16 | >16 | >16 | 0.25 | 0.25 | <0.12 | <0.12 | <0.12 | <0.12 | 0.25 | 2 | 8 | <0.12 | <0.12 | <0.12 | <0.12 | <0.12 | <0.12 | <0.12 | 8 |
| K. pneumoniae NY BS OXA-232 | >16 | 1 | 2 | 16 | >16 | >16 | 0.5 | 1 | <0.12 | <0.12 | <0.12 | <0.12 | 0.5 | 2 | >16 | 0.25 | <0.12 | <0.12 | <0.12 | <0.12 | <0.12 | 0.5 | >16 |
| K. pneumoniae Jefferson 17,73 OXA-181 | >16 | >16 | >16 | >16 | >16 | >16 | 1 | 1 | <0.12 | <0.12 | <0.12 | <0.12 | 1 | 4 | >16 | 0.25 | 0.25 | 0.5 | 0.5 | 1 | <0.12 | 1 | 16 |
| E. coli Jefferson 17,75 OXA-48 | >16 | >16 | >16 | >16 | >16 | 8 | <0.12 | <0.12 | <0.12 | <0.12 | <0.12 | <0.12 | <0.12 | 0.25 | 2 | <0.12 | <0.12 | <0.12 | <0.12 | <0.12 | <0.12 | <0.12 | 2 |
| Ceftazidime-Avibactam-Resistant Clinical P. aeruginosa Isolates28 | |||||||||||||||||||||||
| P. aeruginosa CL232 | >16 | >16 | >16 | >16 | >16 | >16 | >16 | >16 | 8 | 4 | 16 | 16 | >16 | 16 | >16 | >16 | >16 | >16 | 16 | >16 | >16 | >16 | >16 |
| P. aeruginosa 715 | >16 | >16 | 16 | >16 | >16 | >16 | 16 | >16 | 8 | 0.25 | 8 | 16 | 16 | 16 | >16 | >16 | 16 | 16 | 0.5 | 16 | 8 | 16 | 16 |
| P. aeruginosa 776 | >16 | >16 | 8 | >16 | >16 | >16 | >16 | >16 | 16 | 0.25 | 16 | 16 | >16 | 16 | >16 | 16 | >16 | 16 | 16 | 16 | 16 | >16 | >16 |
| P. aeruginosa 795 | >16 | 16 | 4 | >16 | >16 | >16 | >16 | >16 | 0.5 | <0.12 | 16 | 16 | >16 | >16 | 16 | 16 | 16 | 4 | <0.12 | 16 | 4 | 16 | 8 |
| P. aeruginosa 839 | >16 | 8 | 4 | >16 | >16 | 8 | 8 | 8 | <0.12 | <0.12 | 2 | 0.5 | 4 | 4 | 1 | 1 | 1 | <0.12 | <0.12 | 0.5 | <0.12 | 1 | 1 |
| Clinical A. baumannii Isolates Producing blaOXA-23 and/or blaOXA-24 | |||||||||||||||||||||||
| A. baumannii PR319 OXA-23 and OXA- 24/40 | >16 | >16 | >16 | >16 | >16 | >16 | >16 | >16 | >16 | >16 | >16 | >16 | >16 | >16 | >16 | >16 | >16 | >16 | >16 | >16 | >16 | >16 | >16 |
| A. baumannii PR323 OXA-24/40 | >16 | >16 | >16 | >16 | >16 | >16 | >16 | >16 | 4 | 8 | 4 | 4 | >16 | >16 | >16 | 16 | 16 | >16 | >16 | >16 | >16 | >16 | >16 |
| A. baumannii PR359 OXA-23 | >16 | >16 | >16 | >16 | >16 | 16 | 8 | 8 | 8 | 16 | 8 | 16 | 16 | 16 | >16 | 4 | 4 | >16 | >16 | >16 | >16 | >16 | >16 |
| A. baumannii PR363 OXA-23 | >16 | >16 | >16 | >16 | >16 | 16 | 8 | 8 | 8 | 8 | 8 | 8 | >16 | 16 | >16 | 4 | 2 | >16 | >16 | >16 | >16 | >16 | >16 |
| A. baumannii PR381 OXA-24/40 | >16 | >16 | >16 | >16 | >16 | >16 | 16 | 16 | 16 | 16 | 16 | 16 | >16 | >16 | >16 | >16 | >16 | >16 | >16 | >16 | >16 | >16 | >16 |
| A. baumannii PR408 OXA-24/40 | >16 | >16 | >16 | >16 | >16 | 16 | 8 | 8 | 16 | 16 | 8 | 16 | >16 | 16 | >16 | >16 | >16 | >16 | >16 | >16 | >16 | >16 | >16 |
| Clinical A. baumannii Isolates Producing blaOXA-23 and/or blaOXA-24 | |||||||||||||||||||||||
| A. baumannii PR420 OXA-23 and OXA- 24/40 | >16 | >16 | >16 | >16 | >16 | >16 | >16 | >16 | >16 | >16 | >16 | >16 | >16 | >16 | >16 | 16 | 8 | >16 | >16 | >16 | >16 | >16 | >16 |
| A. baumannii PR440 OXA-23 and OXA- 24/40 | >16 | >16 | >16 | >16 | >16 | >16 | 16 | >16 | >16 | >16 | 16 | >16 | 16 | >16 | >16 | 4 | 2 | >16 | >16 | >16 | >16 | >16 | >16 |
| A. baumannii PR448 OXA-23 | >16 | >16 | >16 | >16 | >16 | 8 | 2 | 4 | 4 | 8 | 4 | 8 | 16 | 16 | >16 | 2 | 1 | >16 | >16 | >16 | >16 | >16 | >16 |
| A. baumannii PR474 OXA-23 and OXA- 24/40 | >16 | >16 | >16 | >16 | >16 | >16 | 8 | 8 | 8 | 8 | 8 | 8 | >16 | >16 | >16 | >16 | 16 | >16 | >16 | >16 | >16 | >16 | >16 |
| A. baumannii PR476 OXA-24/40 | >16 | >16 | >16 | >16 | >16 | 1 | 2 | 2 | 1 | 2 | 1 | 2 | 2 | 2 | 0.25 | 0.25 | 0.25 | 0.25 | 0.25 | 0.25 | 0.25 | 0.25 | 0.25 |
| A. baumannii AADC pWH1266 OXA-24/40 | >16 | >16 | >16 | >16 | >16 | >16 | 16 | 16 | 16 | 16 | 16 | 16 | >16 | >16 | >16 | >16 | >16 | >16 | >16 | >16 | >16 | >16 | >16 |
a
DBOs were used at either 4 μg/mL (−4) or 8 μg/mL (−8).