Table 2.
Primer sequences used for real-time PCR amplification of selected genes in the livers of lactating goats
| Genea | Primers 5′ to 3’b | Size(bp) | Accession c |
|---|---|---|---|
| SOD1 | ACACAAGGCTGTACCAGTGC TTCACATTGCCCAGGTCTCC |
104 | NM_001285550.1 |
| SOD2 | TCAATAAGGAGCAGGGACGC AGCAGGGGGATAAGACCTGT |
85 | XM_005684984.1 |
| SOD3 | ATCGACCCGAACGGTAACGC AGGACATAGAAGGGGTCTGCG |
199 | XM_005681678.1 |
| CAT | ACAATGTCACTCAGGTGCGG TCTCACACAGGCGTTTCCTC |
70 | XM_005690077.1 |
| GPx1 | GTAGCATCGCTCTGAGGCAC TTGGCATTTTCCTGATGCCC |
131 | XM_005695962.1 |
| GPx3 | TTCGGTCTGGTCATTCTGGG CGAACATACTTGAGGGTGGC |
95 | XM_005683183.1 |
| HMOX2 | AGCTAGACAAAGGTGCCCTG CAGGATGCTGACAGGCAAAG |
113 | XM_005697930.1 |
| MGST3 | AGCTCACCAGAACACGTTGG GCCCAAACCAGAAGCTATGC |
94 | NM_001285615.1 |
| NQO1 | CATGGCTGTCAGAAAAGCACTG GACACAGTGACCTCCCATCC |
120 | XM_005692193.1 |
| UGT1A1 | TCCGGAGCAGAAAGCTATGG CCGAGTCTTTGGGTGACCAAG |
160 | XM_005678746.1 |
| TXNRD1 | TTTCGCTCAGTTTGCTCCAG TCCTGAGAAGCCTTCAGGGTC |
200 | XM_005680564.1 |
| NF-kB | AGGTGGCGATCGTTGTTCTA TTGCCTTTGTTCTTCCTGCC |
226 | XM_00569893.1 |
| NrF2 | AGCCAGGTGAGATGGAACTG CCAGACTCCCTGTTTCGCTG |
120 | XM_005679848.2 |
| MT2A | TCCTGCAAGAAGAGCTGCTG AACTGCACTTGTCCGAGGC |
94 | XM_005691999.1 |
| SRXN1 | GACACGATCCGGGAGAATCC GGTCTGAGAGGGTGGATTGG |
175 | XM_013968785.1 |
| MT1A | ATAATAGCGCTCGGCTCCTG TTGGAGGAAAAGCGAGGTCC |
98 | XM_005692001.2 |
| GAPDH | CCTGCCCGTTCGACAGATAG CCGTTCTCTGCCTTGACTGT |
249 | XM_005680968.2 |
aSOD1 superoxide dismutase 1, SOD2 superoxide dismutase 2, SOD3 superoxide dismutase3, GPX1 glutathione peroxidase1, GPX3 glutathione peroxidase 3, CAT catalase, HMOX2 haem oxygenase 2, MGST3 microsomal glutathione S-transferase 3, MT1A metallothionein 1A, NQO1 NAD(P)H, quinone 1, UGT1A1 UDP glucuronosyltransferase 1 family, polypeptide A1, MT2A metallothionein 2A, TXNRD1 thioredoxin reductase 1, SRXN1 sulfiredoxin 1, GAPDH glyceraldehyde 3-phosphate dehydrogenase
bThe first primer listed for each gene is the forward primer, and the second primer is the reverse primer
cThe reference sequence numbers are given for primers whose source is the National Center for Biotechnology Information (NCBI) GenBank database (http://www.ncbi.nlm.nih.gov/genbank/)