Fig. 6.
α-Syn reduces the expression of β-arrestin2 in astrocyte. a–b α-Syn increased Drd2 expression in astrocytes. The expression of Drd2 in astrocytes treated with wide-type (WT) α-Syn or from A53T transgenic (A53Ttg/tg) mice were evaluated by the qPCR analysis (a) and Western Blot analysis (b). Data are presented as the mean ± S.E.M from four independent experiments, t test, *p < 0.05 vs. control group. c α-Syn failed to influence the binding ability of Drd2 using radioligand binding analysis. d α-Syn did not affect the suppressive effects of quinpirole on intracellular cAMP concentration using a cAMP assay kit. Data are presented as the mean ± S.E.M from four independent experiments, two-way ANOVA, **p < 0.01 vs. corresponding control group, ##p < 0.01 vs. corresponding forskolin treatment group. e–f α-Syn reduced the expression of β-arrestin2 in astrocytes. Representative immunoblot and quantitative analysis of β-arrestin2 in astrocytes from A53T transgenic (A53Ttg/tg) mice (e) and treated with wide-type (WT) α-Syn (f). Data are presented as the mean ± S.E.M from four independent experiments, two-way ANOVA, *p < 0.05 vs. control group