Table 1.

Primer information and standard curves of microflora for Q-PCR

Bacterial species	Primer sequence (5 → 3)	Regression curve/Tm	Reference
Total bacteria	F: CGGYCCAGACTCCTACGGG	y = 14.354–0.2607X	[20]
	R: TTACCGCGGCTGCTGGCAC	R2 = 0.997 Tm = 59.5 °C
Firmicutes	F: GGAGYATGTGGTTTAATTCGAAGCA	y = 13.073–0.2985X	[21]
	R: AGCTGACGACAACCATGCAC	R2 = 0.998 Tm = 60.5 °C
Bacteroidetes	F: GGARCATGTGGTTTAATTCGATGAT	y = 14.390–0.2807X	[21]
	R: AGCTGACGACAACCATGCAG	R2 = 0.998 Tm = 58 °C
Bifidobacterium	F: TCGCGTCYGGTGTGAAAG	y = 13.837–0.2045X	[21]
	R: CCACATCCAGCRTCCAC	R2 = 0.998 Tm = 61.5 °C
Enterococcus spp.	F: CCCTTATTGTTAGTTGCCATCATT	y = 14.356–0.2542X	[21]
	R: ACTCGTTGTACTTCCCATTGT	R2 = 0.998 Tm = 52 °C
Lactobacillus spp.	F: AGCAGTAGGGAATCTTCCA	y = 14.899–0.2863X	[22]
	R: CACCGCTACACATGGAG	R2 = 0.999 Tm = 58 °C
A. hydrophila	F: GAAAGGTTGATGCCTAATACGTA	y = 13.903–0.2050×
	R: CGTGCTGGCAACAAAGGACAG	R2 = 0.991 Tm = 53.5 °C
Enterobacteriaceae	F: CATTGACGTTACCCGCAGAAGAAGC	y = 13.870–0.1927X	[23]
	R: CTCTACGAGACTCAAGCTTGC	R2 = 0.995 Tm = 53 °C
Streptococcus spp.	F: AGAGTTTGATCCTGGCTCAG	y = 14.086–0.2630X	[24]
	R: GTTAGCCGTCCCTTTCTGG	R2 = 0.996 Tm = 59.5 °C

Note: “X” is representative the value of “Ct” of PCR, “C” representative the “Cycle”, “t” representative the “threshold”. And “y” is representative the Log10 DNA gene copies quantification data